Effects of heparan-like polymers associated with growth factors on osteoblast proliferation and phenotype expression

摘要: Heparan-like polymers derived from dextran, named RGTA, were shown to stimulate bone repair in dif- ferent defect models. Like heparin and heparan sul- fates, RGTA potentiate vitro the biological activities of heparin-binding growth factors (HBGFs), such as fibroblast factor (FGF), by stabilizing them against denatur- ations enhancing their binding with cellular recep- tors. postulated healing interacting HBGFs released wound site and, sub- sequently, promoting proliferation and/or differen- tiation cells implicated this process. We examined effects alone associated on MC3T3-E1 osteoblastic cell differentiation. inhibited proliferation, measured (3H)- thymidine incorporation into DNA. They enhanced in- hibition DNA synthesis caused transforming factor-b (TGF-b1) morphogenetic protein-2 (BMP- 2). increased alkaline phosphatase parathyroid hormone-responsive adenylate cyclase activi- ties MC3T3. stimulation alka- line activity induced BMP-2 decreased or suppressed inhibition TGF-b1 FGF-2. Furthermore, response hormone stimulated BMP-2. In conclusion, stimu- late expression osteoblast phenotype features association HBGFs. The ability promote ferentiation bone-forming is a potential explanation stimulating effect repair. © 1999 John Wiley & Sons, Inc. J Biomed Mater Res, 44, 63-72, 1999.