Population structure of Sclerotinia sclerotiorum on sunflower in Australia

作者: M. G. Ekins , H. L. Hayden , E. A. B. Aitken , K. C. Goulter

DOI: 10.1007/S13313-010-0018-6

关键词: Genetic diversityHelianthus annuusSclerotinia sclerotiorumBiologyVeterinary medicineSunflowerBotanyGenotypeAscusRoot rotRestriction fragment length polymorphism

摘要: Sunflower (Helianthus annuus) is host to infections by Sclerotinia sclerotiorum originating from either homothallic sexually-derived ascospores (stem and head rots) or asexually-derived sclerotia (root rot). While sunflower can be infected this study found no association between the genotypes in lesions type of infection (stem, root Multicopy Restriction Fragment Length Polymorphisms (RFLPs) showed individual comprised only one genotype, that all eight within an ascus also had genotype. Mycelial Compatibility Groups (MCGs), Random Amplified Polymorphic DNAs (RAPDs), single multicopy RFLP analyses majority plants were A sample 250 isolates collected hierarchically sunflowers Queensland New South Wales shown belong large genetic population S. sclerotiorum. Temporal studies revealed uniformity was maintained across years, further confirming population. range molecular markers used genotype 120 isolates, resulting differing levels resolution a Between 13 24 identified with similarities differences assemblages each depending on marker used.

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