A test of the human sperm acrosome reaction following ionophore challenge. Relationship to fertility and other seminal parameters.
作者： Peter E. Hartmann , James M. Cummins , Sandra M. Pember , John L. Yovich , Anne M. Jequier
DOI: 10.1002/J.1939-4640.1991.TB00223.X
关键词: Ionophore 、 Dimethyl sulfoxide 、 Semen 、 Sperm 、 Andrology 、 Population 、 Human fertilization 、 Biology 、 Acrosome reaction 、 Acrosome
摘要: Acrosome reaction capacity was tested on semen samples from 53 fertile and 26 subfertile men. Preparations were divided into two aliquots after 3 or 24 hours of culture. One aliquot received 10 mumol/L calcium ionophore A23187 in dimethyl sulfoxide (DMSO) the other DMSO alone. reactions scored ethanol-permeabilized smears using fluorescein isothiocyanate (FITC)-conjugated Pisum sativum lectin. The following factors analyzed: spontaneous rates (control); induced (ionophore-challenged); difference between two, being proportion spermatozoa population capable reacting response to influx (acrosome challenge [ARIC]). While bore no relation fertility, ARICs significantly reduced absent men, indicating acrosomal dysfunction as a likely cause fertilization failure. test shown have predictive value for fertility comparable that hamster ovum sperm penetration assay be simple cost-effective addition existing semenology.
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