Direct Intramuscular Injection of Plasmid DNA Encoding Angiopoietin-1 but not Angiopoietin-2 Augments Revascularization in the Rabbit Ischemic Hindlimb
作者： Kou-Gi Shyu , Orit Manor , Meredith Magner , George D. Yancopoulos , Jeffrey M. Isner
DOI: 10.1161/01.CIR.98.19.2081
关键词: Hindlimb 、 Pathology 、 Endocrinology 、 Neovascularization 、 Genetic transfer 、 Medicine 、 Angiopoietin receptor 、 Saline 、 Internal medicine 、 Ischemia 、 Lagomorpha 、 Collateral circulation
摘要: Background—Angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2) have recently been identified as ligands for the endothelial cell–specific Tie2 receptor. Little is known regarding impact of these on postnatal neovascularization. Accordingly, we tested hypothesis that gene transfer plasmid DNA encoding Ang1 Ang2 could modulate collateral vessel development in a rabbit model hindlimb ischemia. Methods Results—pAng1* (n=15), pJFE control (no Ang1* insert) (n=9), pAng2 pcDNA3 (n=10), or saline (n=5) was injected intramuscularly into ischemic hindlimb. Collateral limb perfusion were assessed before 30 days after treatment. Calf blood pressure ratio (ischemic to normal hindlimb) increased versus controls (Ang1*, 0.90±0.02; pJFE, 0.76±0.05; saline, 0.77±0.03; P<0.05). Angiographic score higher (P<0.05) pAng1* group (0.63±0.02) than (0.51±0.03) ...
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