Atomic Force Microscopy Study of the Adhesion of Saccharomyces cerevisiae.

摘要: Abstract An atomic force microscope (AFM) has been used to quantify directly the adhesion of metabolically active Saccharomyces cerevisiae cells at a hydrophilic mica surface, surface with hydrophobic coating, and protein-coated in an aqueous environment. The measurements “cell probes” constructed by immobilizing single cell apex tipless AFM cantilever. Adhesion was quantified from force–distance data for retraction surface. indicated stretching sequential bond-breaking as probe retracted all surfaces. Detailed studies were made physiologically cells, which shown have different properties glutaraldehyde-treated cells. Greatest measured Prior adsorption bovine serum albumin protein layer did not significantly affect adhesion. Changes yeast hydrophobicity zeta-potential age correlated differences Cells stationary phase adhered most strongly Time contact demonstrated be important. Both needed detach length adhesive interaction increased after 5 min contact. technique gives unique insights into primary colonization events biofilm formation. It will continue aid both fundamental assessment new procedures that are designed lower surfaces relevant biotechnology, medicine, dentistry