Titanium dioxide nanoparticle-induced testicular damage, spermatogenesis suppression, and gene expression alterations in male mice.
作者： Guodong Gao , Yuguan Ze , Xiaoyang Zhao , Xuezi Sang , Lei Zheng
DOI: 10.1016/J.JHAZMAT.2013.04.046
关键词: Gene expression 、 Biology 、 Endocrinology 、 Sperm 、 Internal medicine 、 Toxicity 、 Reproductive toxicity 、 Gene expression profiling 、 Microarray analysis techniques 、 Spermatogenesis 、 Hormone metabolism
摘要: Although titanium dioxide nanoparticles (TiO2 NPs) have been demonstrated to accumulate in organs resulting toxicity, there is currently only limited data regarding male reproductive toxicity by TiO2 NPs. In this study, testicular damage and alterations gene expression profiles mice induced intragastric administration of 2.5, 5, 10mg/kg body weight NPs for 90 consecutive days were examined. Our findings showed that can cross the blood-testis barrier reach testis therein, which, turn, results lesions, sperm malformations, serum sex hormone levels. Furthermore, microarray analysis 70 genes with known functions up-regulated, while 72 down-regulated NPs-exposed testes. Of altered expressions, Ly6e, Adam3, Tdrd6, Spata19, Tnp2, Prm1 are involved spermatogenesis, whereas Sc4mol, Psmc3ip, Mvd, Srd5a2, Lep, Cyp2e1 associated steroid metabolism. Hence, production application should be carried out cautiously, especially humans age.
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sci-hub.se 参考文章(62)
Jesus M. Torres, José A. Gómez‐Capilla, Estrella Ruiz, Esperanza Ortega, Semiquantitative RT-PCR method coupled to capillary electrophoresis to study 5alpha-reductase mRNA isozymes in rat ventral prostate in different androgen status. Molecular and Cellular Biochemistry. ,vol. 250, pp. 125- 130 ,(2003) , 10.1023/A:1024902419502
Toshinobu Miyamoto, Kazuo Sengoku, Shiga Hasuike, Naoyuki Takuma, Hiroaki Hayashi, Tsuyoshi Yamashita, Mutsuo Ishikawa, Isolation and Expression Analysis of the Human Testis-Specific Gene, SPERGEN-1, a Spermatogenic Cell-Specific Gene-1 Journal of Assisted Reproduction and Genetics. ,vol. 20, pp. 101- 104 ,(2003) , 10.1023/A:1021748310102
S Andersson, R W Bishop, D W Russell, Expression cloning and regulation of steroid 5α-reductase, an enzyme essential for male sexual differentiation Journal of Biological Chemistry. ,vol. 264, pp. 16249- 16255 ,(1989) , 10.1016/S0021-9258(18)71614-1
Zhang TianBao, Wan XuYing, Xu GuiFeng, Hou Juan, Wang Fei, Liu Zhen, Effects of titanium dioxide nanoparticles on development and maturation of rat preantral follicle in vitro. Academic Journal of Second Military Medical University. ,vol. 30, pp. 869- 873 ,(2009)
S. Ghafouri-Fard, A. Abbasi, H. Moslehi, N. Faramarzi, S. Taba taba Vakili, M.B. Mobasheri, M.H. Modarressi, Elevated expression levels of testis-specific genes TEX101 and SPATA19 in basal cell carcinoma and their correlation with clinical and pathological features British Journal of Dermatology. ,vol. 162, pp. 772- 779 ,(2010) , 10.1111/J.1365-2133.2009.09568.X
Janet Estee Kacharmina, Peter B. Crino, James Eberwine, Preparation of cDNA from single cells and subcellular regions. Methods in Enzymology. ,vol. 303, pp. 3- 18 ,(1999) , 10.1016/S0076-6879(99)03003-7
Juan HOU, Xu-ying WAN, Fei WANG, Gui-feng XU, Zhen LIU, Tian-bao ZHANG, Effects of titanium dioxide nanoparticles on development and maturation of rat preantral follicle in vitro : Effects of titanium dioxide nanoparticles on development and maturation of rat preantral follicle in vitro Academic Journal of Second Military Medical University. ,vol. 29, pp. 869- 873 ,(2009) , 10.3724/SP.J.1008.2009.00869
Mihoko Hosokawa, Masanobu Shoji, Kouichi Kitamura, Takashi Tanaka, Toshiaki Noce, Shinichiro Chuma, Norio Nakatsuji, Tudor-related proteins TDRD1/MTR-1, TDRD6 and TDRD7/TRAP: domain composition, intracellular localization, and function in male germ cells in mice. Developmental Biology. ,vol. 301, pp. 38- 52 ,(2007) , 10.1016/J.YDBIO.2006.10.046
Doni Hikmat Ramdhan, Yuki Ito, Yukie Yanagiba, Nozomi Yamagishi, Yumi Hayashi, ChunMei Li, Shinji Taneda, Akira K. Suzuki, Gen Watanabe, Kazuyoshi Taya, Michihiro Kamijima, Tamie Nakajima, Nanoparticle-rich diesel exhaust may disrupt testosterone biosynthesis and metabolism via growth hormone Toxicology Letters. ,vol. 191, pp. 103- 108 ,(2009) , 10.1016/J.TOXLET.2009.08.013
Jiangxue Wang, Chunying Chen, Ying Liu, Fang Jiao, Wei Li, Fang Lao, Yufeng Li, Bai Li, Cuicui Ge, Guoqiang Zhou, Yuxi Gao, Yuliang Zhao, Zhifang Chai, None, Potential neurological lesion after nasal instillation of TiO2 nanoparticles in the anatase and rutile crystal phases Toxicology Letters. ,vol. 183, pp. 72- 80 ,(2008) , 10.1016/J.TOXLET.2008.10.001