Semiquantitative RT-PCR method coupled to capillary electrophoresis to study 5alpha-reductase mRNA isozymes in rat ventral prostate in different androgen status.

摘要: The development and growth of the prostate gland depends on androgen stimulation. Dihydrotestosterone (DHT) is primary responsible for also pathogenesis benign prostatic hyperplasia (BPH). incidence cancer (PCa) hypertrophy (BPH) continues to rise in Western world. DHT synthesized from circulating testosterone (T) through action 5α-Reductase (5α-R) (EC 1.3.99.5), which occurs as two isozymes, type 1 2. Type-1 5α-R widely distributed body, type-2 confined androgen-dependent structures. Both types are expressed prostate: isozyme implicated BPH PCa; type-1 increased some adenocarcinomas. In recent years, various inhibitors or both have been used diseases. this work we present measurements mRNA levels steroid isozymes ventral rats different status. We a novel method that combines high specificity semiquantitive PCR with sensitivity laser-induced capillary electrophoresis (LIF-CE). demonstrated T control expression 5α-R2 rat prostrate. This approach could be great value study diseases humans would allow at transcriptional level effects drugs inhibit either these isozymes.