Prognostic implications of ploidy and proliferative activity in human solid tumors.

摘要: Ploidy and cell cycle compartment distribution were measured by DNA flow cytometry in 261 patients with a variety of different tumors. Eighty-one percent all tumors aneuploid, 72% hyperdiploid. levels spanned wide range from hypodiploid (maximum 30% < diploid controls) to hyperoctaploid (440% excess mean median values coinciding at near-triploid content. The proportion cells G1 content decreased increasing hyperdiploid abnormality. While unrelated biopsy site number host factors such as age, sex race, both ploidy cytokinetic parameters markedly affected histopathologic diagnosis. Patients metastatic lung, breast, GI cancer had higher than individuals the corresponding primary (except for one patient) remained constant, G1/0 proportions showed only minor variation disease over observation time 6 months. Prognostic factor analysis was performed subgroup studied within months adverse impact low tumor on survival lost proportional hazard extended include diagnostic subgroups. Accounting diagnosis, stage disease, ploidy, cells, following sequence prognostic order their relative ranks established: (1) absence breast (p = 0.001), (2) hypertriploid index 0.049), (3) presence 0.079). Our study demonstrates that content-derived information instrinsic features pertaining cytogenetics cytokinetics may provide an objective means biologically relevant classification.