Identification of two testosterone-responsive testicular proteins in Sertoli cell-enriched culture medium whose secretion is suppressed by cells of the intact seminiferous tubule.

摘要: Of 30 proteins identified in medium from primary Sertoli cell-enriched cultures, five of these appear to increase primarily response testosterone. Using high performance liquid chromatography, two proteins, designated CMB-22 and CMB-23, were purified apparent homogeneity medium. Both are monomeric with molecular weights Mr 37,000 40,000, respectively. interact concanavalin A wheat germ agglutinin on lectin blots. has a pI 5.8; CMB-23 distinctive isoelectric variants pIs 5.4 5.2, the latter variant was Isoform. Polyvalent antisera raised against rabbits cross-reacted one another. Removal carbohydrate by either enzymatic or chemical treatments reduced their but did not abolish size differences sodium dodecyl sulfate-polyacrylamide gels. Peptide maps generated Staphylococcus aureus protease V8 visualized silver staining immunoblots suggest that similar share almost identical epitopes. radioimmunoassay developed used measure total immunoreactive CMB-22-like material (CMB-22 plus immunoreactivity) culture media, biological fluids, tissue extracts. The results studies showed secretion is unique regard other have been cultures. That is, secreted cannot be detected media cultures intact tubular segments vitro. In addition, also testicular fluids interstitium, tubule, rete testis. This striking contrast cell which present substantial concentrations fluids. These observations possibly hormone responsiveness normally suppressed tubule both vivo We propose will provide important insights into cell-cell interactions seminiferous epithelium.