3D printed conformal microfluidics for isolation and profiling of biomarkers from whole organs

摘要: The ability to interface microfluidic devices with native complex biological architectures, such as whole organs, has the potential shift paradigm for study and analysis of tissue. Here, we show 3D printing can be used fabricate bio-inspired conformal that directly surface organs. Structured-light scanning techniques enabled topographical matching device geometry porcine kidney anatomy. Our studies molecular species are spontaneously transferred from organ cortex in presence fluid flow through organ-conforming microchannel. Large animal using kidneys (n = 32 organs) revealed profile stream was dependent on preservation conditions. Enzyme-linked immunosorbent assay (ELISA) isolate clinically relevant metabolic pathophysiological biomarkers including heat shock protein 70 (HSP-70) injury molecule-1 (KIM-1), which were detected high 409 12 pg mL−1, respectively. Overall, these results enable a novel minimally invasive ‘microfluidic biopsy’ technique isolation profiling organs within interval. This achievement could assessment, thereby helping relieve shortage crisis increased availability quality donor Ultimately, this work provides major advance microfluidics design manufacturing microfluidic-based