Reaction Mechanism of 17β-Estradiol Dehydrogenase Determined by Equilibrium Rate Exchange
作者： George Betz
DOI: 10.1016/S0021-9258(19)77189-0
关键词: NAD+ kinase 、 Chemical transformation 、 Reaction mechanism 、 Steroid 、 Cofactor 、 Stereochemistry 、 Estradiol Dehydrogenases 、 Substrate (chemistry) 、 Chemistry 、 Binary complex
摘要: Abstract The reaction mechanism of human placental estradiol dehydrogenase (17β-estradiol:NAD-oxidoreductase, EC 1.1.1.62) has been investigated by measuring the rate isotopic exchange between substrate-product pairs while varying concentrations unlabeled reactants. results show that is random with respect to order substrate addition and binary complex formation involving either cosubstrate or steroid may occur. Substitution NADP:NADPH for NAD:NADH produces a marked increase in rates suggesting decrease Michaelis constants. Inequality indicates chemical transformation not rate-limiting step over-all reaction. No abortive was detected.
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