Site specificity and mechanism of human placental 17β-hydroxysteroid dehydrogenase
作者： James C. Warren , Robert D. Crist
DOI: 10.1016/0003-9861(67)90392-X
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摘要: Abstract Kinetic analysis of the site specificity purified human placental 17β-hydroxysteroid dehydrogenase in terms cofactor (NAD + and NADP ) steroid (estradiol-17β estradiol 3-sulfate) indicate that estradiol-17β 3-sulfate bind at a single activ-site. While NAD have an active common, second binding (active or allosteric) lower affinity is also present. Product inhibition studies are compatible with Ordered Bi mechanism first Iso Theorell-Chance first.
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