Role of Histone Metabolism and Chromatin Structure in DNA Repair

摘要: During the cell cycle progression, synthesis of new histones is important to pack newly synthesized DNA and maintain proper chromatin structure. Unlike normal mRNAs, mRNAs from replication-dependent histone genes that are expressed in S phase not polyadenylated at 3‘end. They contain a conserved stem-loop sequence which forms structure required for processing 3’end, translation degradation mRNAs. However, number studies show can produce have polyA tail 3’end under certain conditions physiological function clear. In present study, we analyzed expression H2B genes. Furthermore, HIST1H2BD HIST1H2AC up-regulated during differentiation up on induction damage. We showed transported cytoplasm form polysomes suggesting theses transcripts be translated into proteins. In addition proteins, post-translational modifications, ATP-dependent remodelers chaperones play roles maintaining genome controlling associated processes such as replication, transcription repair. Here one monoubiquitination (H2Bub1) was shown with actively transcribed genes, double strand break (DSB) H2Bub1 carried out by an E3 ubiquitin ligase complex RNF20/40 knockdown RNF40 leads loss checkpoint activation. addition, also regulates recruitment chaperone FACT dynamics DSB site. Further, CHD1 helicase recruited site binding CtIP chromatin. Depletion causes decrease homologous recombination-mediated repair efficiency increase cellular sensitivity Mitomycin C treatment. In summary, data imply remodeler mediate through remodeling