CHD1 interacts with SSRP1 and depends on both its chromodomain and its ATPase/helicase-like domain for proper association with chromatin.

摘要: CHD1, an M r∼200,000 protein that contains a chromodomain (C), ATPase/helicase-like domain (H) and DNA-binding (D), was previously shown to be associated with decompacted interphase chromatin in mammalian cells transcriptionally active puffs interbands Drosophila polytene chromosomes. We now show by transient transfection experiments genes expressing wild-type mutant forms of CHD1 both the C H domains are essential for its proper association chromatin. also present evidence vivo interaction between novel HMG box-containing protein, SSRP1, which involves amino-terminal segment does not include chromodomain. Immunocytochemical analyses indicated SSRP1 colocalize nuclei