Identification of a putative Bacillus subtilis rho gene.

摘要: Transposon Tn917 mutagenesis of Bacillus subtilis BD99 followed by selection for protonophore resistance led to the isolation strain MS119, which contained a single insertion in an open reading frame whose deduced amino acid sequence was 56.6% identical that Escherichia coli rho gene product. The insertional site near beginning frame, located region B. chromosome spoOF gene; new data several frames surrounding putative are presented. predicted Rho protein would have 427 acids and molecular weight 48,628. growth mutant less than wild type on defined medium at 30 degrees C. On yeast extract-supplemented medium, MS119 comparable C but much slower lower temperatures; sporulation occurred competence developed cells grown To determine whether sensitivity low temperature resulted from insertion, chloramphenicol cassette inserted into wild-type BD170; resulting derivative displayed same phenotype as MS119.