The effect of histone deacetylase inhibition on the expression of P-glycoprotein in human placental trophoblast cell lines.
作者： Hongyu Duan , Chuan Wang , Kaiyu Zhou , Tao Wang , Yifei Li
DOI: 10.1016/J.PLACENTA.2016.11.011
关键词: Trophoblast 、 Messenger RNA 、 Trichostatin A 、 Epigenetics 、 Molecular biology 、 Histone deacetylase 、 Placenta 、 Real-time polymerase chain reaction 、 HDAC1 、 Biology
摘要: Abstract Introductions Placental P-glycoprotein (P-gp), encoded by ABCB1 gene in human, plays a significant role regulating drugs' transplacental transfer rates. Investigations on placental P-gp regulation could provide more therapeutic targets for individualized and safe pharmacotherapy during pregnancy. Currently, the epigenetic control of is rare. This study aimed to investigate effect histone deacetylases (HDACs) inhibition expression trophoblast cell lines explore whether HDAC1/2/3 was involved this process preliminarily. Methods Human (Bewo JAR) were treated with two different HDAC inhibitors-suberoylanilide hydroxamic acid (SAHA) trichostatin A (TSA) at concentration gradients 0.5, 1.0, 3.0 5.0 μM. Cells harvested after 24, 48, 72 h treatment. Total activity detected colorimetric assay Kits. HDAC1/2/3/ABCB1 mRNA protein expressions determined real-time quantitative PCR western-blot, respectively. Pearson correlation analysis test performed relationship between expression. Results SAHA TSA inhibit total both Bewo JAR, but displayed transient induction or level being low dosage prolonged exposure drugs. Discordance also observed. significantly induced company HDACs inhibition. There negative linear HDAC1/2 Conclusions up-regulate cells, most likely be process.
sci-hub.se 参考文章(37)
Paulus, Pharmacotherapy in pregnancy Therapeutische Umschau. Revue thérapeutique. ,vol. 56, pp. 602- 607 ,(1999) , 10.1024/0040-5930.56.10.602
Aizati N. A. Daud, Jorieke E. H. Bergman, Marian K. Bakker, Hao Wang, Wilhelmina S. Kerstjens-Frederikse, Hermien E. K. de Walle, Henk Groen, Jens H. J. Bos, Eelko Hak, Bob Wilffert, P-Glycoprotein-Mediated Drug Interactions in Pregnancy and Changes in the Risk of Congenital Anomalies: A Case-Reference Study Drug Safety. ,vol. 38, pp. 651- 659 ,(2015) , 10.1007/S40264-015-0299-3
MK Bakker, J Jentink, F Vroom, PB Van Den Berg, HEK De Walle, LTW De Jong-Van Den Berg, Maternal medicine: Drug prescription patterns before, during and after pregnancy for chronic, occasional and pregnancy-related drugs in the Netherlands BJOG: An International Journal of Obstetrics & Gynaecology. ,vol. 113, pp. 559- 568 ,(2006) , 10.1111/J.1471-0528.2006.00927.X
Shengkan Jin, Kathleen W Scotto, None, Transcriptional Regulation of the MDR1 Gene by Histone Acetyltransferase and Deacetylase Is Mediated by NF-Y Molecular and Cellular Biology. ,vol. 18, pp. 4377- 4384 ,(1998) , 10.1128/MCB.18.7.4377
Gerda Lagger, Dónal O'Carroll, Martina Rembold, Harald Khier, Julia Tischler, Georg Weitzer, Bernd Schuettengruber, Christoph Hauser, Reinhard Brunmeir, Thomas Jenuwein, Christian Seiser, Essential function of histone deacetylase 1 in proliferation control and CDK inhibitor repression The EMBO Journal. ,vol. 21, pp. 2672- 2681 ,(2002) , 10.1093/EMBOJ/21.11.2672
S.G. Matthews, P. Lye, E. Bloise, C. Dunk, M. Javam, W. Gibb, S.J. Lye, Effect of oxygen on multidrug resistance in the first trimester human placenta Placenta. ,vol. 34, pp. 817- 823 ,(2013) , 10.1016/J.PLACENTA.2013.05.010
Kanako Wakabayashi-Nakao, Ai Tamura, Tomoka Furukawa, Hiroshi Nakagawa, Toshihisa Ishikawa, Quality control of human ABCG2 protein in the endoplasmic reticulum: Ubiquitination and proteasomal degradation Advanced Drug Delivery Reviews. ,vol. 61, pp. 66- 72 ,(2009) , 10.1016/J.ADDR.2008.08.008
Lesli Ann Rawles, Diana Acuna, Robert P. Erickson, The role of multiple drug resistance proteins in fetal and/or placental protection against teratogen-induced orofacial clefting. Molecular Reproduction and Development. ,vol. 74, pp. 1483- 1489 ,(2007) , 10.1002/MRD.20734
Chuan Wang, Kaiyu Zhou, Liang Xie, Yifei Li, Yalan Zhan, Lina Qiao, Chaoyi Qin, Ruiqi Liu, Yimin Hua, Maternal medication use, fetal 3435 C>T polymorphism of the ABCB1 gene, and risk of isolated septal defects in a Han Chinese population. Pediatric Cardiology. ,vol. 35, pp. 1132- 1141 ,(2014) , 10.1007/S00246-014-0906-6
Renata Silva, Vânia Vilas-Boas, Helena Carmo, Ricardo Jorge Dinis-Oliveira, Félix Carvalho, Maria de Lourdes Bastos, Fernando Remião, Modulation of P-glycoprotein efflux pump: induction and activation as a therapeutic strategy Pharmacology & Therapeutics. ,vol. 149, pp. 1- 123 ,(2015) , 10.1016/J.PHARMTHERA.2014.11.013