In Silico Assigned Resistance Genes Confer Bifidobacterium with Partial Resistance to Aminoglycosides but Not to Β-Lactams

摘要: Bifidobacteria have received significant attention due to their contribution human gut health and the use of specific strains as probiotics. It is thus not surprising that there has also been interest with respect antibiotic resistance profile. Numerous culture-based studies demonstrated bifidobacteria are resistant majority aminoglycosides, but sensitive β-lactams. However, limited research exists genetic basis for aminoglycosides. Here we performed an in-depth in silico analysis putative Bifidobacterium-encoded aminoglycoside proteins β-lactamases assess these resistance. The silico-based screen detected β-lactam across Bifidobacterium genus. Laboratory-based investigations a number representative confirmed despite containing β-lactamases, were In contrast, all aminoglycosides tested. To genes encoding sp. two genes, namely Bbr_0651 Bbr_1586, targeted insertional inactivation B. breve UCC2003. As compared wild-type, UCC2003 insertion mutant exhibited decreased gentamycin, kanamycin streptomycin. This study highlights associated risks relying on assignment gene function. Although several located bifidobacteria, presence does coincide antibiotics. contrast however, this approach resulted identification loci contribute and, potentially, many other bifidobacteria.