Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains

摘要: The display of proteins on the surface phage offers a powerful means selecting for rare genes encoding with binding activities. Recently we found that antibody heavy and light chain variable (V) domains fused as single polypeptide to minor coat protein filamentous fd, could be enriched by successive rounds growth panning antigen. This allows selection antigen-binding directly from diverse libraries V-genes. Now show heterodimeric Fab fragments can assembled linking one protein, secreting other into bacterial periplasm. Furthermore introducing an amber mutation between either using supE strains bacteria, or produce soluble fragment non-suppressor strains. use may offer advantages over Fv construction combinatorial libraries.