Fermentative production of self-toxic fungal secondary metabolites

摘要: Fungi are well known for their vast diversity of secondary metabolites that include many life-saving drugs and highly toxic mycotoxins. In general, fungal cultures producing such immune to effects. However, some produce self-toxic compounds can pose production optimization challenges if the needed in large amounts chemical modification. One culture, LV-2841, was identified as lead one our exploratory projects. This culture found be a slow grower produced trace metabolite, cercosporamide, under standard flask fermentation conditions, extensive medium studies failed yield higher titers. Poor growth liquid media attributed self-toxicity cercosporamide organism, minimum inhibitory concentration (MIC) estimated range 8–16 μg/ml. Fermentations carried out containing Diaion® HP20 resin afforded significantly titers desired compound. While several examples resin-based fermentations soil streptomyces have been published, this approach has rarely used fermentations. Over 100-fold increase titer achieved by supplementing with commercially available neutral adsorbent resin.