A transient expression system to assay putative antifungal genes on powdery mildew infected barley leaves

摘要: A transient expression system has been established to assay the regulation and impact of putative antifungal proteins at single cell level in barley (Hordeum vulgareL.) epidermal leaf cells infected with powdery mildew (Erysiphe graminisf.sp.hordei).The combines biolistic transformation direct comparison fungal attack transfected wild type cells. Many showed detectable marker genes including a novel improved version green fluorescent protein (GFP). The was for least 10 days during which individual host supported an unaffected asexual life-cycle pathogen.