Quantification of differences between measured values and statistical validation based on the differences
作者： Bartlomiej P. Rajwa , Joseph P. Robinson , Tytus S. Bernas
DOI:
关键词: Mathematics 、 Statistics 、 Matrix (mathematics) 、 Measure (mathematics) 、 Biological system 、 Statistical validation 、 Statistical hypothesis testing 、 Histogram 、 Metric (mathematics) 、 Calibration
摘要: A system and method of validating differences between measured values fluorescence intensities obtained from a fluorescence-based instrument, including: generating calibration histograms for beads run through the instrument; calibrating distance function configured to measure distances by: constructing metric using function, which includes bin-to-bin dissimilarity matrix; populating matrix maximize following conditions: (A) two representing sets different equals difference their MESF values; (B) identical is zero; applying flow cytometry generated biological samples, determine histograms; statistical test significance distances; determining whether histogram results samples are reliable based on significance.
lens.org 参考文章(11)
Mingyong Han, Xiaohu Gao, Jack Z. Su, Shuming Nie, Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules Nature Biotechnology. ,vol. 19, pp. 631- 635 ,(2001) , 10.1038/90228
Jonas F. Dorn, Gaudenz Danuser, Ge Yang, Computational processing and analysis of dynamic fluorescence image data. Methods in Cell Biology. ,vol. 85, pp. 497- 538 ,(2008) , 10.1016/S0091-679X(08)85022-4
Abraham Schwartz, Alan D. Hetzel, Automated system for performance analysis and fluorescence quantitation of samples ,(1993)
Mario Roederer, Adam Treister, Wayne Moore, Leonore A. Herzenberg, Probability binning comparison: a metric for quantitating univariate distribution differences. Cytometry. ,vol. 45, pp. 37- 46 ,(2001) , 10.1002/1097-0320(20010901)45:1<37::AID-CYTO1142>3.0.CO;2-E
C. Faloutsos, R. Barber, M. Flickner, J. Hafner, W. Niblack, D. Petkovic, W. Equitz, Efficient and effective querying by image content intelligent information systems. ,vol. 3, pp. 231- 262 ,(1994) , 10.1007/BF00962238
Tytus Bernas, Elikplimi K. Asem, J. Paul Robinson, Bartek Rajwa, Quadratic form: a robust metric for quantitative comparison of flow cytometric histograms. Cytometry Part A. ,vol. 73, pp. 715- 726 ,(2008) , 10.1002/CYTO.A.20586
Santiago Costantino, Christopher B. Kent, Antoine G. Godin, Timothy E. Kennedy, Paul W. Wiseman, Alyson E. Fournier, Semi-automated quantification of filopodial dynamics. Journal of Neuroscience Methods. ,vol. 171, pp. 165- 173 ,(2008) , 10.1016/J.JNEUMETH.2008.02.009
Jonathan W.D. Comeau, Santiago Costantino, Paul W. Wiseman, A Guide to Accurate Fluorescence Microscopy Colocalization Measurements Biophysical Journal. ,vol. 91, pp. 4611- 4622 ,(2006) , 10.1529/BIOPHYSJ.106.089441
Volodymyr Kudryavtsev, Suren Felekyan, Anna K. Woźniak, Marcelle König, Carl Sandhagen, Ralf Kühnemuth, Claus A. M. Seidel, Filipp Oesterhelt, Monitoring dynamic systems with multiparameter fluorescence imaging Analytical and Bioanalytical Chemistry. ,vol. 387, pp. 71- 82 ,(2006) , 10.1007/S00216-006-0917-0
Santiago Costantino, Jonathan W.D. Comeau, David L. Kolin, Paul W. Wiseman, Accuracy and Dynamic Range of Spatial Image Correlation and Cross-Correlation Spectroscopy Biophysical Journal. ,vol. 89, pp. 1251- 1260 ,(2005) , 10.1529/BIOPHYSJ.104.057364