Signature molecular changes in the skeletal muscle of hindlimb unloaded mice.
作者： Muhammad Azeem , Rizwan Qaisar , Asima Karim , Anu Ranade , Adel Elmoselhi
DOI: 10.1016/J.BBREP.2021.100930
关键词: Catabolism 、 Asparagine 、 Hydroxyproline 、 Endoplasmic reticulum 、 Skeletal muscle 、 Biophysics 、 Muscle atrophy 、 Hindlimb 、 Glutamine 、 Chemistry
摘要: Abstract Hind-limb unloaded (HU) mouse is a well-recognized model of muscle atrophy; however, the molecular changes in skeletal during unloading are poorly characterized. We have used Raman spectroscopy to evaluate structure and behavior signature molecules involved regulating structural functional health. The spectroscopic analysis gastrocnemius muscles was compared between 16-18 weeks old HU c57Bl/6J mice ground-based controls. spectra showed that signals for asparagine glutamine were reduced mice, possibly indicating increased catabolism. peaks hydroxyproline proline split, pointing towards breakdown tendon repair. also report consistently intensity in> 1300 cm-1 range along with shift higher frequencies activation sarcoplasmic reticulum (SR) stress HU.
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