Identification and characterization of a heat-resistant protease from Serratia liquefaciens isolated from Brazilian cold raw milk.
作者： Solimar Gonçalves Machado , Marc Heyndrickx , Jan De Block , Bart Devreese , Isabel Vandenberghe
DOI: 10.1016/J.IJFOODMICRO.2016.01.014
关键词: Pseudomonas 、 Proteolytic enzymes 、 Cold storage 、 Raw milk 、 Biology 、 Food microbiology 、 Food spoilage 、 Serratia liquefaciens 、 Psychrotrophic bacteria 、 Microbiology
摘要: The cold storage of raw milk before heat treatment in dairy industry promotes the growth psychrotrophic microorganisms, which are known for their ability to produce heat-resistant proteolytic enzymes. Although Pseudomonas is described as main causative genus high spoilage potential products, Serratia liquefaciens secretes proteases and may be found samples well. However, at present there no information about S. after heat-treatment. aim this research was assess isolated from Brazilian characterize involved protease. shown secrete one metalloprotease of, approximately, 52 kDa encoded by ser2 gene. heat-resistance Ser2 similar aprX produced Pseudomonas. gene detected all isolates tested study, activity highly heterogeneous. Since nucleotide deduced amino acid sequences identical, heterogeneity attributed differences enzyme expression levels or post-translational modifications.
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