Pseudomonasspp. andSerratia liquefaciensas Predominant Spoilers in Cold Raw Milk
作者： Solimar G. Machado , Fernanda L. da Silva , Denise M.S. Bazzolli , Marc Heyndrickx , Paulo M. de A. Costa
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摘要: The storage of fresh raw milk at low temperature does not prevent proliferation psychrotrophic bacteria that can produce heat-resistant proteolytic enzymes contributing to the reduced shelf life dairy products. This study aimed identify dominant enzyme-producing population from Brazil. Raw samples collected in 3 different cooling tanks Brazil were stored optimal (45 h 4 °C followed by 7 °C) and suboptimal 10 conditions simulate farm transportation allowed Brazilian laws. highly strains isolated cold characterized repetitive sequence-based Polymerase Chain Reaction (PCR) analysis. clustering resulted 8 clusters solitary fingerprints. most isolates each rep-cluster selected for identification using miniaturized kit, 16S rDNA rpoB gene sequencing. Serratia liquefaciens (73.9%) Pseudomonas spp. (26.1%) identified as microorganisms with high spoilage potential. knowledge microbiota will contribute improved quality
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