Use of the rpoB gene as an alternative to the V3 gene for the identification of spoilage and pathogenic bacteria species in milk and milk products
作者： V. Deperrois-Lafarge , T. Meheut
DOI: 10.1111/J.1472-765X.2012.03261.X
关键词:
摘要: Aim: To evaluate the rpoB gene as an alternative to V3 for identification of bacterial species in milk and products. Methods Results: DNA obtained from different strains was amplified by PCR using primers. products each were then separated on a DGGE gel. The molecular fingerprints tested integrated into database. analysis shows single band amplicons per species. Comparison electrophoretic profiles 16S rDNA amplification with those this study showed that some co-migrated after region, distinct bands observed gel region. Conclusions: results show discriminatory power gene, indicating it can be used rRNA products. Significance Impact Study: PCR-DGGE targeting is way discriminating so avoids sequencing co-migrating bands.
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