Stem cell-mediated accelerated bone healing observed with in vivo molecular and small animal imaging technologies in a model of skeletal injury.
作者： Sheen-Woo Lee , Parasuraman Padmanabhan , Pritha Ray , Sanjiv Sam Gambhir , Timothy Doyle
DOI: 10.1002/JOR.20736
关键词: Multipotent Stem Cell 、 Stem cell 、 Bioluminescence imaging 、 Adult stem cell 、 Biology 、 In vivo 、 Bone Injury 、 Pathology 、 Bone healing 、 Bone regeneration
摘要: Adult stem cells are promising therapeutic reagents for skeletal regeneration. We hope to validate by molecular imaging technologies the in vivo life cycle of adipose-derived multipotent (ADMCs) an animal model injury. Primary ADMCs were lentivirally transfected with a fusion reporter gene and injected intravenously into mice bone injury or sham operation. Bioluminescence (BLI), [(18)F]FHBG (9-(fluoro-hydroxy-methyl-butyl-guanine)-micro-PET, [(18)F]Fluoride ion micro-PET micro-CT performed monitor their effect. microscopy immunohistochemistry done histological confirmation. BLI showed ADMC's traffic from lungs then site. confirmed defect. Micro-CT measurements increased healing cell-injected group compared noninjected at postoperative day 7 (p < 0.05). Systemically administered site facilitate healing, as demonstrated small imaging. Molecular can usage adult adipose tissue-derived promote fracture healing. Imaging future help establish strategies including dosage administration route.
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