Structure of the TFIIIC subcomplex τ A provides insights into RNA polymerase III pre-initiation complex formation
作者： Matthias K. Vorländer , Anna Jungblut , Kai Karius , Florence Baudin , Helga Grötsch
DOI: 10.1101/2020.04.17.046805
关键词: RNA polymerase 、 Cell biology 、 RNA polymerase III 、 RNA polymerase II 、 Gene 、 CTCF 、 Chromatin 、 Protein subunit 、 Transcription factor 、 Chemistry
摘要: Transcription factor (TF) IIIC is a conserved eukaryotic six-subunit protein complex with dual function. It serves as general TF for most RNA polymerase (Pol) III genes by recruiting TFIIIB, but it also involved in chromatin organization and regulation of Pol II through interaction CTCF condensin II. Here, we report the structure S. cerevisiae TFIIIC subcomplex τA, which contains subunits responsible recruitment TFIIIB transcription start site (TSS) selection at genes. We show that τA binding to its promoter auto-inhibited disordered acidic tail subunit τ95. further provide negative stain reconstruction bound Brf1 TBP an unexpected location TBP. This shows ruler element achieves positioning upstream TSS, suggests remodeling during assembly TFIIIC.
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