Borosilicate Glass Fiber-Optic Biosensor for the Detection of Escherichia coli.

摘要: Polyclonal antibodies against Escherichia coli and fluorescent, secondary, were immobilized on borosilicate glass fibers pre-treated with 3-glycidyloxypropyl trimethoxysilane (GPS). Light an average wavelength of 627 nm, emitted by a diode placed at one end the fiber, was detected ultrasensitive photodiode peak sensitivity 640 nm. Changes in fluorescence, caused binding E. to antibodies, changed net refractive index fiber thus internal reflection light. These evanescent changes photon energy recorded photodiode. Signals amplified voltage digital multimeter. A linear increase readings over 2 h when 3.0 × 107 CFU/ml 2.77 109 adhered antibodies. Voltage cell numbers from 103 106 CFU/ml, but remained unchanged for h, indicating that limit detection is CFU/ml. This simple technology may be used develop low-cost, portable, fiber-optic biosensor detect infections have applications medical field. Research progress optimize determine its specificity.