The Coordination Chemistry of DNA Nucleosides on Gold Nanoparticles as a Probe by SERS

摘要: The DNA nucleosides (dA, dC, dG, dT) bound to gold nanoparticles (~13 nm) in aqueous solution has been studied as a probe by the SERS and their coordination structures have proposed on basis of them. According UV-Visible absorption after modifying with nucleosides, rates dA, dG were much faster than that dT monitored aggregation kinetics at 700 nm. These data indicated had higher affinity for surface nucleoside dT. As result spectra, binding modes each assigned. A dA binds via N(7) nitrogen atom imidazole ring, which C(6)-NH2 group also participates process. In case it N(3) pyrimidine ring partial contribution from oxygen C(2)=O group. surfaces is proposed. Although two different nitrogens amino group, N(1) hydrogen migrates Conversely, C(4)=O ring. Accordingly, these suggest or will bind more fast carbonyl