Matrix-free LDI mass spectrometry platform using patterned nanostructured gold thin film.

摘要: A novel matrix-free LDI MS platform using a thin film of patterned nanostructured gold, capped with methyl- and carboxy-terminated self-assembled monolayers (SAMs) is presented. Calibration on the surface was performed peptide standard mixture available for MALDI analysis. analysis limit detection angiotensin I peptide. Peptide fragments from protein digests bovine serum albumin, catalase, lactoperoxidase were used to carry out mass fingerprinting Sequence coverage each digest number detected compared conventional plate. Versatility gold substrate illustrated by performing different enzymes small molecule