A polymerase chain reaction method for detecting dwarf mistletoe infection in douglas-fir and western larch.
作者： M Marler , D Pedersen , T Mitchell-Olds , R M Callaway
DOI: 10.1139/X99-092
关键词: Parasitic plant 、 Arceuthobium douglasii 、 Polymerase chain reaction 、 Botany 、 Arceuthobium 、 Loranthaceae 、 Larix occidentalis 、 Parasite hosting 、 Virology 、 DNA 、 Biology
摘要: Early detection and management of dwarf mistletoe (Arceuthobium spp.) is currently limited by the inability to rapidly detect infection during 2- 5-year endophyte phase parasite. We describe a polymerase chain reaction (PCR) technique for detecting Arceuthobium douglasii Engelm. laricis in tissues its hosts, Pseudotsuga menziesii (Mirb.) Franco Larix occidentalis Nutt. DNA was extracted from branches 15 infected uninfected P. menziesii. The PCR product amplified using specific primer rbcL gene template fragment 708 pairs bases length. This all that were visibly infected, but not generated any samples known be uninfected. conifer 385 length pure DNA; this as an internal positive control. primers developed me...
mpg.de
core.ac.uk
cabdirect.org
nrcresearchpress.com参考文章(11)
Carleton B. Edminster, Robert L. Mathiasen, Frank G. Hawksworth, EFFECTS OF DWARF MISTLETOE ON GROWTH AND MORTALITY OF DOUGLAS-FIR IN THE SOUTHWEST The Great Basin naturalist. ,vol. 50, pp. 173- 179 ,(1990)
Robert L. Mathiasen, Dwarf mistletoes in forest canopies Northwest Science. ,vol. 70, pp. 61- 71 ,(1996)
K Groppe, T Boller, PCR assay based on a microsatellite-containing locus for detection and quantification of Epichloë endophytes in grass tissue. Applied and environmental microbiology. ,vol. 63, pp. 1543- 1550 ,(1997) , 10.1128/AEM.63.4.1543-1550.1997
Monique Gardes, Thomas J. White, J. André Fortin, Thomas D. Bruns, John W. Taylor, Identification of indigenous and introduced symbiotic fungi in ectomycorrhizae by amplification of nuclear and mitochondrial ribosomal DNA Botany. ,vol. 69, pp. 180- 190 ,(1991) , 10.1139/B91-026
Peter Wyss, Paola Bonfante, Amplification of genomic DNA of arbuscular-mycorrhizal (AM) fungi by PCR using short arbitrary primers Fungal Biology. ,vol. 97, pp. 1351- 1357 ,(1993) , 10.1016/S0953-7562(09)80169-X
Robin M. Reich, Paul W. Mielke Jr., Frank G. Hawksworth, Spatial analysis of ponderosa pine trees infected with dwarf mistletoe Canadian Journal of Forest Research. ,vol. 21, pp. 1808- 1815 ,(1991) , 10.1139/X91-249
Ed F. Wicker, James M. Wells, Intensification and lateral spread of Arceuthobiumlaricis in a young stand of western larch with stocking control Canadian Journal of Forest Research. ,vol. 13, pp. 314- 319 ,(1983) , 10.1139/X83-044
M. E. Devey, K. D. Jermstad, C. G. Tauer, D. B. Neale, Inheritance of RFLP loci in a loblolly pine three-generation pedigree Theoretical and Applied Genetics. ,vol. 83, pp. 238- 242 ,(1991) , 10.1007/BF00226257
J. P. CLAPP, J. P. W. YOUNG, J. W. MERRYWEATHER, A. H. FITTER, Diversity of fungal symbionts in arbuscular mycorrhizas from a natural community New Phytologist. ,vol. 130, pp. 259- 265 ,(1995) , 10.1111/J.1469-8137.1995.TB03047.X
Delbert Wiens, Frank G. Hawksworth, Dwarf mistletoes : biology, pathology, and systematics Agricultural Handbook 709. Washington, D.C.: U.S. Dept. of Agriculture, Forest Service. 410 p.. ,(1996)