Identification of a Novel 70kDa Protein in Cultured Tobacco Cells That is Immunologically Related to MAP4.
作者： Tetsuya Higashiyama , Seiji Sonobe , Hiromu Murofushi , Seiichiro Hasezawa
关键词: Bovine adrenal 、 Cell biology 、 Microtubule 、 Biology 、 Antibody 、 Epitope 、 Biochemistry 、 HSPA2
摘要: We fractionated heat-stable proteins co-purified with microtubules from tobacco BY-2 cells. One of them, a 70 kDa protein, cross-reacted antibodies against bovine adrenal MAP4. It was also revealed that common epitopes exist in the amino-terminal projection domain MAP4, at end opposite microtubule-binding domain.
sci-hub.se 参考文章(20)
Toshiyuki Nagata, Kazuya Okada, Itaru Takebe, Chiaki Matsui, Delivery of tobacco mosaic virus RNA into plant protoplasts mediated by reverse-phase evaporation vesicles (Liposomes) Molecular and General Genetics MGG. ,vol. 184, pp. 161- 165 ,(1981) , 10.1007/BF00272899
H. Aizawa, Y. Emori, A. Mori, H. Murofushi, H. Sakai, K. Suzuki, Functional analyses of the domain structure of microtubule-associated protein-4 (MAP-U). Journal of Biological Chemistry. ,vol. 266, pp. 9841- 9846 ,(1991) , 10.1016/S0021-9258(18)92896-6
S Kotani, H Murofushi, S Maekawa, H Aizawa, H Sakai, Isolation of rat liver microtubule-associated proteins. Evidence for a family of microtubule-associated proteins with molecular mass of around 200,000 which distribute widely among mammalian cells. Journal of Biological Chemistry. ,vol. 263, pp. 5385- 5389 ,(1988) , 10.1016/S0021-9258(18)60728-8
H Aizawa, H Kawasaki, H Murofushi, S Kotani, K Suzuki, H Sakai, A common amino acid sequence in 190-kDa microtubule-associated protein and tau for the promotion of microtubule assembly. Journal of Biological Chemistry. ,vol. 264, pp. 5885- 5890 ,(1989) , 10.1016/S0021-9258(18)83632-8
H. Aizawa, Y. Emori, H. Murofushi, H. Kawasaki, H. Sakai, K. Suzuki, Molecular cloning of a ubiquitously distributed microtubule-associated protein with Mr 190,000. Journal of Biological Chemistry. ,vol. 265, pp. 13849- 13855 ,(1990) , 10.1016/S0021-9258(18)77426-7
J.C. Bulinski, G.G. Borisy, Microtubule-associated proteins from cultured HeLa cells. Analysis of molecular properties and effects on microtubule polymerization Journal of Biological Chemistry. ,vol. 255, pp. 11570- 11576 ,(1980) , 10.1016/S0021-9258(19)70328-7
L M Parysek, C F Asnes, J B Olmsted, MAP 4: occurrence in mouse tissues. Journal of Cell Biology. ,vol. 99, pp. 1309- 1315 ,(1984) , 10.1083/JCB.99.4.1309
James A. Weatherbee, Ronald B. Luftig, Robert R. Weihing, Purification and reconstitution of HeLa cell microtubules. Biochemistry. ,vol. 19, pp. 4116- 4123 ,(1980) , 10.1021/BI00558A033
Marylin Vantard, Paul Schellenbaum, Arlette Fellous, Anne Marie Lambert, Characterization of maize microtubule-associated proteins, one of which is immunologically related to tau. Biochemistry. ,vol. 30, pp. 9334- 9340 ,(1991) , 10.1021/BI00102A028
Robert W. Woody, David C. Clark, Gordon C. K. Roberts, Stephen R. Martin, Peter M. Bayley, Molecular flexibility in microtubule proteins: proton nuclear magnetic resonance characterization. Biochemistry. ,vol. 22, pp. 2186- 2192 ,(1983) , 10.1021/BI00278A020