Transport and programmed release of nanoscale cargo from cells by using NETosis.

摘要: Cells can take up nanoscale materials, which has important implications for understanding cellular functions, biocompatibility as well biomedical applications. Controlled uptake, transport and triggered release of cargo is one the great challenges in applications nanomaterials. Here, we study how human immune cells (neutrophilic granulocytes, neutrophils) nanomaterials program them to this after a certain time period. For purpose, let neutrophils phagocytose DNA-functionalized single-walled carbon nanotubes (SWCNTs) vitro that fluoresce near infrared (980 nm) serve sensors small molecules. still migrate, follow chemical gradients respond inflammatory signals uptake cargo. To release, make use neutrophil extracellular trap formation (NETosis), novel cell death mechanism leads chromatin swelling, subsequent rupture membrane cell's whole content. By using process NETosis, point via initial concentration stimuli such phorbol 12-myristate-13-acetate (PMA) or lipopolysaccharide (LPS). At intermediate stimulation, continue surface cues around 30 minutes several hundred micrometers until they stop SWCNTs. The transported released SWCNT are functional shown by detection neurotransmitter dopamine reactive oxygen species (H2O2). In summary, hijack biological (NETosis) demonstrate