Oridonin inhibits VEGF‑A‑associated angiogenesis and epithelial‑mesenchymal transition of breast cancer in vitro and in vivo

摘要: Metastasis is the primary cause of mortality in patients with breast cancer and lacks effective therapeutic agents. Oridonin, an active diterpenoid compound isolated from Rabdosia rubescens, was identified to be most potent anti-tumor ingredient. However, molecular mechanisms responsible for its anti-metastatic effects remain unclear. In present study, oridonin significantly suppressed migration, invasion adhesion MDA-MB-231 4T1 cells, inhibited tube formation human umbilical vein endothelial cells a dose-dependent manner. The expression levels epithelial-mesenchymal transition (EMT)-associated marker hypoxia inducible factor 1α (HIF-1α)/vascular endothelium growth (VEGF) signaling pathway mRNA proteins were determined by reverse transcription-quantitative polymerase chain reaction western blotting, respectively vitro. results demonstrated that effectively EMT as significant increases E-cadherin, decreased N-cadherin, Vimentin Snail. addition, exerted anti-angiogenesis activity through decreasing HIF-1α, VEGF-A VEGF receptor-2 protein expression. Furthermore, decrease micro-vessel density evidenced cluster differentiation 31, neovasculature. brief, inhibits tumor cell adhesion, well angiogenesis, which are mediated suppressing HIF-1α/VEGF pathway. study suggest may promising agent treatment.