Proteomic Analysis of Clathrin Interactions in Trypanosomes Reveals Dynamic Evolution of Endocytosis

摘要: Endocytosis is a vital cellular process maintaining the cell surface, modulating signal transduction and facilitating nutrient acquisition. In metazoa, multiple endocytic modes are recognized, but for many unicellular organisms likely dominated by ancient clathrin-mediated pathway. The system of highly divergent trypanosomatid Trypanosoma brucei exhibits unusual features, including restricted site internalization, dominance plasma membrane GPI-anchored proteins, absence AP2 complex an exceptionally high rate. Here we asked if proteins subtending clathrin trafficking in trypanosomes exclusively related to those higher eukaryotes or novel, potentially taxon-specific operate. Co-immunoprecipitation identified twelve T. clathrin-associating (TbCAPs), which partially colocalized with clathrin. Critically, eight TbCAPs genomes all these required robust proliferation. A subset, TbCAP100, TbCAP116, TbCAP161 TbCAP334, were implicated distinct steps detailed analysis knockdown cells. Coupled orthologs metazoan fungal factors, data suggest that interactions lineage-specific, indicate substantial evolutionary diversity within endocytosis mechanisms across eukaryotes.