Plasmodium falciparum: Cloning by single-erythrocyte micromanipulation and heterogeneity in vitro
作者： Ayoade M.J. Oduola , Norman F. Weatherly , Jean H. Bowdre , Robert E. Desjardins
DOI: 10.1016/0014-4894(88)90053-7
关键词: Population 、 Genetic heterogeneity 、 Protozoa 、 Plasmodium falciparum 、 Parasite hosting 、 In vitro 、 Genetics 、 Biology 、 Phenotype 、 Drug resistance
摘要: Natural isolates of Plasmodium falciparum represent a genetically heterogeneous population parasites. To obtain stable strains the parasites for long term experiments, rapid and definitive method cloning was developed using micropipets micromanipulator. Homogeneous parasite clones prepared by this technique were characterized compared with parent during 4 years continuous culture. The process phenotypic dominance selection drug resistance which occur in nature also simulated vitro evaluating dynamics two cocultured P. falciparum.
elsevier.com
sci-hub.se 参考文章(13)
W Trager, J. Jensen, Human malaria parasites in continuous culture Science. ,vol. 193, pp. 673- 675 ,(1976) , 10.1126/SCIENCE.781840
Sodsri Thaithong, G.H. Beale, B. Fenton, J. McBride, V. Rosario, A. Walker, D. Walliker, Clonal diversity in a single isolate of the malaria parasite Plasmodium falciparum Transactions of The Royal Society of Tropical Medicine and Hygiene. ,vol. 78, pp. 242- 245 ,(1984) , 10.1016/0035-9203(84)90287-6
J. DAVID HAYNES, CARTER L. DIGGS, FRED A. HINES, ROBERT E. DESJARDINS, Culture of human malaria parasites Plasmodium falciparum Nature. ,vol. 263, pp. 767- 769 ,(1976) , 10.1038/263767A0
J. Le Bras, P. Deloron, A. Ricour, B. Andrieu, J. Savel, J.P. Coulaud, Plasmodium falciparum: Drug sensitivity in vitro of isolates before and after adaptation to continuous culture Experimental Parasitology. ,vol. 56, pp. 9- 14 ,(1983) , 10.1016/0014-4894(83)90091-7
W. Trager, M. Tershakovec, L. Lyandvert, H. Stanley, N. Lanners, E. Gubert, Clones of the malaria parasite Plasmodium falciparum obtained by microscopic selection: their characterization with regard to knobs, chloroquine sensitivity, and formation of gametocytes Proceedings of the National Academy of Sciences of the United States of America. ,vol. 78, pp. 6527- 6530 ,(1981) , 10.1073/PNAS.78.10.6527
Michael D. Rogers, Norman F. Weatherly, Jean H. Bowdre, Robert E. Desjardins, End-tidal exhaled air for the in vitro maintenance of Plasmodium falciparum Transactions of the Royal Society of Tropical Medicine and Hygiene. ,vol. 77, pp. 103- 105 ,(1983) , 10.1016/0035-9203(83)90029-9
V Rosario, Cloning of naturally occurring mixed infections of malaria parasites Science. ,vol. 212, pp. 1037- 1038 ,(1981) , 10.1126/SCIENCE.7015505
W K Milhous, N F Weatherly, J H Bowdre, R E Desjardins, In vitro activities of and mechanisms of resistance to antifol antimalarial drugs. Antimicrobial Agents and Chemotherapy. ,vol. 27, pp. 525- 530 ,(1985) , 10.1128/AAC.27.4.525
R E Desjardins, C J Canfield, J D Haynes, J D Chulay, Quantitative assessment of antimalarial activity in vitro by a semiautomated microdilution technique. Antimicrobial Agents and Chemotherapy. ,vol. 16, pp. 710- 718 ,(1979) , 10.1128/AAC.16.6.710
R. Carter, Enzyme variation in Plasmodium berghei and Plasmodium vinckei. Parasitology. ,vol. 66, pp. 297- 307 ,(1973) , 10.1017/S0031182000045236