摘要: Publisher Summary This chapter discusses the techniques to cultivate malarial parasite, Plasmodium falciparum . It also emphasizes on cultivation of erythrocytic stages species other than P.falciparum and in vitro development preerythrocytic sporogonic malaria parasites. The culture medium is Roswell Park Memorial Institute (RPMI)-1640, originally developed for human white blood cells supplemented with 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer hypoxanthine. Human erythrocytes are host cell choice. In nature, P. fulciparum restricted humans. have a longer vivo lifetime those susceptible primates most places easier obtain quantity. cloning method. method permits isolation microscopic examination single infected parasite without use micromanipulator.
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sci-hub.se 参考文章(65)
James B. Jensen, William Trager, Plasmodium Falciparum in Culture: Establishment of Additional Strains* American Journal of Tropical Medicine and Hygiene. ,vol. 27, pp. 743- 746 ,(1978) , 10.4269/AJTMH.1978.27.743
Langreth Sg, Trager W, Reese Rt, Isolation of stages of the human parasite Plasmodium falciparum from culture and from animal blood. Bulletin of The World Health Organization. pp. 53- 61 ,(1979)
William Trager, James B. Jensen, Cultivation of erythrocytic and exoerythrocytic stages of plasmodia. Cultivation of erythrocytic and exoerythrocytic stages of plasmodia.. pp. 271- 319 ,(1980) , 10.1016/B978-0-12-426102-0.50012-9
Arthur W. Rowe, E. Eyster, A. Kellner, Liquid nitrogen preservation of red blood cells for transfusion; a low glycerol-rapid freeze procedure. Cryobiology. ,vol. 5, pp. 119- 128 ,(1968) , 10.1016/S0011-2240(68)80154-3
H. Van den Bossche, Biochemistry of parasites and host-parasite relationships. Biochemistry of parasites and host-parasite relationships.. ,(1976)
W.H. Wernsdorfer, I. McGregor, R. L. Beaudoin, D. Mazier, M. R. Hollingdale, In vitro cultivation of malaria parasites: exoerythrocytic stages. Churchill Livingstone. pp. 307- 320 ,(1988)
C.J. Janse, E.G. Boorsma, J. Ramesar, M.J. Grobbee, B. Mons, Host cell specificity and schizogony of Plasmodium berghei under different in vitro conditions. International Journal for Parasitology. ,vol. 19, pp. 509- 514 ,(1989) , 10.1016/0020-7519(89)90080-5
David A. Foley, Janis Kennard, Jerome P. Vanderberg, Plasmodium berghei: infective exoerythrocytic schizonts in primary monolayer cultures of rat liver cells. Experimental Parasitology. ,vol. 46, pp. 166- 178 ,(1978) , 10.1016/0014-4894(78)90129-7
W Trager, J. Jensen, Human malaria parasites in continuous culture Science. ,vol. 193, pp. 673- 675 ,(1976) , 10.1126/SCIENCE.781840
Susan G. Langreth, Robert T. Reese, Mary R. Motyl, William Trager, Plasmodium falciparum: loss of knobs on the infected erythrocyte surface after long-term cultivation. Experimental Parasitology. ,vol. 48, pp. 213- 219 ,(1979) , 10.1016/0014-4894(79)90101-2