Host cell specificity and schizogony of Plasmodium berghei under different in vitro conditions.

摘要: Abstract Invasion and intra-erythrocytic growth of two strains Plasmodium berghei (ANKA K173) were studied under different in vitro conditions. Some important limiting factors for the mass cultivation this rodent malaria parasite reconsidered. Parasites both developed normally from ringforms into mature schizonts RPMI1640 supplemented with Fetal Calf Serum (FCS). At a temperature 37°C duration schizogonic cycle was comparable to that same parasites developing vivo 27°C, however, asexual took 60–72 h. In medium mouse serum instead FCS severely inhibited. showed strict preference reticulocytes. Red blood cells rats, mice hamsters readily invaded by merozoites strains. Erythrocytes rabbits guinea pigs resistant invasion P. . It is concluded host cell specificity technically limits possibilities The validity recent publications, describing alternative culture systems parasite, discussed.