Effects of intralipid and caffeic acid phenethyl ester on neurotoxicity, oxidative stress, and acetylcholinesterase activity in acute chlorpyriphos intoxication.
作者： Kagan Basarslan , Harun Alp , Serkan Senol , Ibrahim Kaplan , Umit Ozkan
DOI:
关键词: Aché 、 Organophosphate 、 Oxidative stress 、 Biochemistry 、 Medicine 、 Pharmacology 、 Acetylcholinesterase 、 Toxicity 、 Tar (tobacco residue) 、 Neurotoxicity 、 Caffeic acid phenethyl ester
摘要: Chlorpyriphos is one of the most widely used organophosphate (OP) insecticide in agriculture with potential toxicity. Current post-exposure treatments consist anti-cholinergic drugs and oxime compounds. We studied effects intralipid caffeic acid phenethyl ester (CAPE) on chlorpyriphos toxicity to compose an alternative or supportive treatment for OP poisoning. Methods: Forty-nine rats were randomly divided into seven groups. was administered Intralipid (IL) CAPE immediately after chlorpyriphos. Serum acetylcholinesterase (AChE) level, total oxidant status (TOS), antioxidant response (TAR), histologic examination cerebellum brain tissue Hematoxylin-Eosin immunohistochemical dyes examined. Results: enzym levels showed that inhibited AChE while IL alone had no effect, intensifies inhibition effect. Significant difference at between chlorpyriphos+IL chlorpyriphos+CAPE verified has a protective effect inhibition. TAR significantly increased all groups except group, TOS revealed decrease amount oxidative stress. Histologic neuronal degeneration slightly decreased but significant protection degeneration. Conclusion: The results this study gave us three key points. 1) activity important diagnosis intoxication it value determining neuro-degeneration. 2) inhibits may increase muscarinic-nicotinic hyperactivation. Therefore should not be intoxication. 3) decreases severity neurodegeneration symptoms can as agent.
ijcem.com 参考文章(21)
Ahmet Ali Sancaktutar, Necmettin Penbegül, Haluk Söylemez, Osman Evliyaoğlu, Mehmet Tuzcu, Harun Alp, Uğur Fırat, Muhammet Erdal Sak, Mehmet Sıddık Evsen, Effects of malathion in fetal kidney tissues in pregnant rats: teratogenic effects induced by different doses. Kafkas Universitesi Veteriner Fakultesi Dergisi. ,vol. 18, pp. 221- 226 ,(2012)
X. Song, J.D. Violin, F.J. Seidler, T.A. Slotkin, Modeling the developmental neurotoxicity of chlorpyrifos in vitro: macromolecule synthesis in PC12 cells. Toxicology and Applied Pharmacology. ,vol. 151, pp. 182- 191 ,(1998) , 10.1006/TAAP.1998.8424
Ozcan Erel, A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry. ,vol. 38, pp. 1103- 1111 ,(2005) , 10.1016/J.CLINBIOCHEM.2005.08.008
Yusuf Yurumez, Polat Durukan, Yucel Yavuz, Ibrahim Ikizceli, Levent Avsarogullari, Seda Ozkan, Okhan Akdur, Caglar Ozdemir, Acute Organophosphate Poisoning in University Hospital Emergency Room Patients Internal Medicine. ,vol. 46, pp. 965- 969 ,(2007) , 10.2169/INTERNALMEDICINE.46.6304
Nermin Serbecic, Sven Christoph Beutelspacher, Anti-oxidative vitamins prevent lipid-peroxidation and apoptosis in corneal endothelial cells Cell and Tissue Research. ,vol. 320, pp. 465- 475 ,(2005) , 10.1007/S00441-004-1030-3
Azza A. Attia, Reda H. ElMazoudy, Nahla S. El-Shenawy, Antioxidant role of propolis extract against oxidative damage of testicular tissue induced by insecticide chlorpyrifos in rats Pesticide Biochemistry and Physiology. ,vol. 103, pp. 87- 93 ,(2012) , 10.1016/J.PESTBP.2012.04.002
Samuel Peña-Llopis, M.Dolores Ferrando, Juan B Peña, Fish tolerance to organophosphate-induced oxidative stress is dependent on the glutathione metabolism and enhanced by N-acetylcysteine Aquatic Toxicology. ,vol. 65, pp. 337- 360 ,(2003) , 10.1016/S0166-445X(03)00148-6
L Masoud, C Vijayasarathy, M Fernandez-Cabezudo, G Petroianu, AM Saleh, Effect of malathion on apoptosis of murine L929 fibroblasts: a possible mechanism for toxicity in low dose exposure. Toxicology. ,vol. 185, pp. 89- 102 ,(2003) , 10.1016/S0300-483X(02)00596-6
Raja Rezg, Bessem Mornagui, Saloua El-Fazaa, Najoua Gharbi, Caffeic acid attenuates malathion induced metabolic disruption in rat liver, involvement of acetylcholinesterase activity. Toxicology. ,vol. 250, pp. 27- 31 ,(2008) , 10.1016/J.TOX.2008.05.017
Anna Lukaszewicz-Hussain, Role of oxidative stress in organophosphate insecticide toxicity – Short review Pesticide Biochemistry and Physiology. ,vol. 98, pp. 145- 150 ,(2010) , 10.1016/J.PESTBP.2010.07.006