Studies on the mechanisms of autophagy: formation of the autophagic vacuole.
作者： W A Dunn
关键词: Golgi apparatus 、 Autophagosome membrane 、 Cell biology 、 Membrane protein 、 Endosome 、 Vacuole 、 Lysosome 、 Organelle 、 Biology 、 Integral membrane protein
摘要: Autophagic vacuoles form within 15 min of perfusing a liver with amino acid-depleted medium. These are bound by "smooth" double membrane and do not contain acid phosphatase activity. In an attempt to identify the source these vacuoles, I have used morphological techniques combined immunological probes localize specific antigens limiting membranes newly formed or nascent autophagic vacuoles. Antibodies three integral proteins plasma (CE9, HA4, epidermal growth factor receptor) one Golgi apparatus (sialyltransferase) did label Internalized its receptor were found in but present lysosome-like degradative All results suggested that from membrane, apparatus, endosome constituents. Antisera prepared against (14, 25, 40 kD) RER was inner outer almost all addition, ribophorin II identified at many Finally, secretory proteins, rat serum albumin alpha 2u-globulin, localized lumen intramembrane space between some The consistent formation ribosome-free regions RER.
rupress.org
core.ac.uk
jcb.org参考文章(61)
J Bertling, U Pfeifer, A morphometric study of the inhibition of autophagic degradation during restorative growth of liver cells in rats re-fed after starvation. Virchows Archiv B Cell Pathology. ,vol. 24, pp. 109- 120 ,(1977) , 10.1007/BF02889272
U. Pfeifer, Hannelore Bergeest, Evelyn Werder, Inhibited autophagic degradation of cytoplasm during compensatory growth of liver cells after partial hepatectomy. Virchows Archiv B Cell Pathology Including Molecular Pathology. ,vol. 30, pp. 313- 333 ,(1979) , 10.1007/BF02889111
B F Trump, A U Arstila, Studies on cellular autophagocytosis. The formation of autophagic vacuoles in the liver after glucagon administration. American Journal of Pathology. ,vol. 53, pp. 687- 733 ,(1968)
B. F. Trump, J. D. Shelburne, A. U. Arstila, Studies on Cellular Autophagocytosis: The Relationship of Autophagocytosis to Protein Synthesis and to Energy Metabolism in Rat Liver and Flounder Kidney Tubules in Vitro American Journal of Pathology. ,vol. 73, pp. 641- 670 ,(1973)
Masaru HIMENO, Hideo OHHARA, Yasuaki ARAKAWA, Keitaro KATO, Beta-glucuronidase of rat preputial gland. Crystallization, properties, carbohydrate composition, and subunits. Journal of Biochemistry. ,vol. 77, pp. 427- 438 ,(1975) , 10.1093/OXFORDJOURNALS.JBCHEM.A130742
M. A. Williams, Quantitative Methods in Biology ,(1978)
Walter C. Schneider, [99] Determination of nucleic acids in tissues by pentose analysis Methods in Enzymology. ,vol. 3, pp. 680- 684 ,(1957) , 10.1016/S0076-6879(57)03442-4
William A. Dunn, Doris A. Wall, Ann L. Hubbard, Use of isolated, perfused liver in studies of receptor-mediated endocytosis. Methods in Enzymology. ,vol. 98, pp. 225- 241 ,(1983) , 10.1016/0076-6879(83)98151-X
J G Barriocanal, J S Bonifacino, L Yuan, I V Sandoval, Biosynthesis, glycosylation, movement through the Golgi system, and transport to lysosomes by an N-linked carbohydrate-independent mechanism of three lysosomal integral membrane proteins. Journal of Biological Chemistry. ,vol. 261, pp. 16755- 16763 ,(1986) , 10.1016/S0021-9258(18)66630-X
Alan J. Barrett, Heidrun Kirschke, [41] Cathepsin B, cathepsin H, and cathepsin L Methods in Enzymology. ,vol. 80, pp. 535- 561 ,(1981) , 10.1016/S0076-6879(81)80043-2