Mapping protein-DNA interactions using ChIP-sequencing
作者： Charles E. Massie , Ian G. Mills
DOI: 10.1007/978-1-61779-376-9_11
关键词: Microarray 、 Genome 、 Computational biology 、 ChIP-sequencing 、 Chromatin immunoprecipitation 、 Tiling array 、 RIP-Chip 、 Biology 、 Genetics 、 Chromatin 、 Southern blot
摘要: Chromatin immunoprecipitation (ChIP) allows enrichment of genomic regions which are associated with specific transcription factors, histone modifications, and indeed any other epitopes present on chromatin. The original ChIP methods used site-specific PCR Southern blotting to confirm the genome were enriched, a candidate basis. combination tiling arrays (ChIP-chip) allowed more unbiased approach map ChIP-enriched sites. However, limitations microarray probe design number have detrimental impact coverage, resolution, sensitivity, cost whole-genome sets for higher eukaryotes large genomes. high-throughput sequencing technology has comprehensive surveys occupancy, greater lower whole coverage. Herein, we provide comparison platforms survey ChIP-seq analysis tools, discuss experimental design, describe detailed method.Chromatin method.
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