Correlative high-resolution morphologic analysis of the three-dimensional organization of human chromosomes.
作者： Pietro Gobbi , Stefan Thalhammer , Mirella Falconi , Robert W. Stark , Wolfgang M. Heckl
关键词: Resolution (electron density) 、 Scanning electron microscope 、 Nanotechnology 、 Biomedical engineering 、 Correlative 、 Biological specimen 、 Scanning confocal electron microscopy 、 Field electron emission 、 Conductive atomic force microscopy 、 Scanning ion-conductance microscopy 、 Materials science
摘要: A correlative morphologic analysis was carried out on isolated metaphase chromosomes by means of field emission in-lens scanning electron microscopy (FEISEM) and atomic force (AFM). Whereas FEISEM provides ultra-high resolution power allows the surface biological structures free any conductive coating, AFM imaging specimens in ambient as well physiologic conditions. The same samples made possible use electrical light transparent ITO glass specimen holder. Further preparation specific for instrumentation not required. Both techniques show a high correlation respective information, improving their reciprocal significance. In particular, coat represents barrier chromosome spreads it is sensitive to protease treatment. chemical removal this layer permits high-resolution chromatid fibers but at time alters chromosomal dimension after rehydration. level, necessary obtain precise physical mapping genome that new instruments such could offer, requires homogeneously cleaned with grade reproducibility. microscopical approach utilizes completely different probes complementary useful information understanding biological, chemical, characteristics can be applied optimize preparations further improvement knowledge about spatial organization.
tu-darmstadt.de
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