Effect of vitrification on in vitro development and imprinted gene Grb10 in mouse embryos.
作者： Jianfeng Yao , Lixia Geng , Rongfu Huang , Weilin Peng , Xuan Chen
DOI: 10.1530/REP-16-0480
关键词: DNA methylation 、 Blastocyst 、 Genomic imprinting 、 Vitrification 、 Andrology 、 Embryo 、 In vitro fertilisation 、 Epigenetics 、 Regulation of gene expression 、 Biology
摘要: Vitrification of embryos is a routine procedure in IVF (in vitro fertilization) laboratories. In the present study, we aimed to investigate effect vitrification on mouse preimplantation embryo development vitro, and epigenetic status imprinted gene Grb10 embryos. The blastocyst formation rate for vitrified 8-cell was similar non-vitrified embryos, whereas hatching lower than that group. expression level major-type transcript decreased significantly blastocysts compared with vivo blastocysts. Moreover, global DNA methylation blastocysts, at CpG island 1 (CGI1) were also after vitrification. culture condition had no adverse effect, except CGI1. These results suggest may reduce affect Grb10.
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