Dimethyl Sulfoxide Has an Impact on Epigenetic Profile in Mouse Embryoid Body
作者： Misa Iwatani , Kohta Ikegami , Yuliya Kremenska , Naka Hattori , Satoshi Tanaka
DOI: 10.1634/STEMCELLS.2005-0427
关键词:
摘要: Dimethyl sulfoxide (DMSO), an amphipathic molecule, is widely used not only as a solvent for water-insoluble substances but also cryopreservant various types of cells. Exposure to DMSO sometimes causes unexpected changes in cell fates. Because mammalian development and cellular differentiation are controlled epigenetically by DNA methylation histone modifications, likely affects the epigenetic system. The effects on transcription three major methyltransferases (Dnmts) five well-studied modification enzymes were examined mouse embryonic stem cells embryoid bodies (EBs) reverse transcription-polymerase chain reaction. Addition (0.02%-1.0%) EBs culture induced increase Dnmt3a mRNA levels with increasing dosage. Increased expression two subtypes protein was confirmed Western blotting. Southern blot analysis revealed that caused hypermethylation kinds repetitive sequences EBs. Furthermore, restriction landmark genomic scanning, which status can be analyzed thousands loci genic regions, affected at multiple loci, inducing hypomethylation well depending loci. In conclusion, has impact profile: upregulation alteration genome-wide profiles phenotypic
elsevier.com
sci-hub.se 参考文章(52)
Robert L. Sinsheimer, The action of pancreatic deoxyribonuclease. II. Isomeric dinucleotides. Journal of Biological Chemistry. ,vol. 215, pp. 579- 583 ,(1955) , 10.1016/S0021-9258(18)65979-4
Peter A. Jones, Stephen B. Baylin, The fundamental role of epigenetic events in cancer Nature Reviews Genetics. ,vol. 3, pp. 415- 428 ,(2002) , 10.1038/NRG816
Taiping Chen, En Li, Structure and function of eukaryotic DNA methyltransferases. Current Topics in Developmental Biology. ,vol. 60, pp. 55- 89 ,(2004) , 10.1016/S0070-2153(04)60003-2
M K Edwards, J F Harris, M W McBurney, Induced muscle differentiation in an embryonal carcinoma cell line. Molecular and Cellular Biology. ,vol. 3, pp. 2280- 2286 ,(1983) , 10.1128/MCB.3.12.2280
Wenzheng ZHANG, Yoshihide HAYASHIZAKI, Bruce C. KONE, Structure and regulation of the mDot1 gene, a mouse histone H3 methyltransferase. Biochemical Journal. ,vol. 377, pp. 641- 651 ,(2004) , 10.1042/BJ20030839
Mona D. Shahbazian, Huda Y. Zoghbi, Rett syndrome and MeCP2: linking epigenetics and neuronal function. American Journal of Human Genetics. ,vol. 71, pp. 1259- 1272 ,(2002) , 10.1086/345360
Jun Ohgane, Teruhiko Wakayama, Sho Senda, Yukiko Yamazaki, Kimiko Inoue, Atsuo Ogura, Joel Marh, Satoshi Tanaka, Ryuzo Yanagimachi, Kunio Shiota, The Sall3 locus is an epigenetic hotspot of aberrant DNA methylation associated with placentomegaly of cloned mice. Genes to Cells. ,vol. 9, pp. 253- 260 ,(2004) , 10.1111/J.1356-9597.2004.00720.X
Jesus Espada, Esteban Ballestar, Mario F. Fraga, Ana Villar-Garea, Angeles Juarranz, Juan C. Stockert, Keith D. Robertson, François Fuks, Manel Esteller, Human DNA methyltransferase 1 is required for maintenance of the histone H3 modification pattern. Journal of Biological Chemistry. ,vol. 279, pp. 37175- 37184 ,(2004) , 10.1074/JBC.M404842200
M Okano, Dnmt2 is not required for de novo and maintenance methylation of viral DNA in embryonic stem cells Nucleic Acids Research. ,vol. 26, pp. 2536- 2540 ,(1998) , 10.1093/NAR/26.11.2536
D. Humpherys, Epigenetic Instability in ES Cells and Cloned Mice Science. ,vol. 293, pp. 95- 97 ,(2001) , 10.1126/SCIENCE.1061402