Human DNA methyltransferase 1 is required for maintenance of the histone H3 modification pattern.
作者： Jesus Espada , Esteban Ballestar , Mario F. Fraga , Ana Villar-Garea , Angeles Juarranz
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摘要: DNA methyltransferase 1 (DNMT1) plays an essential role in murine development and is thought to be the enzyme primarily responsible for maintenance of global methylation status genomic DNA. However, loss DNMT1 human cancer cells affects only a limited number pericentromeric sequences. Here we show that lacking display at least two important differences with respect wild type cells: profound disorganization nuclear architecture, altered pattern histone H3 modification results increase acetylation decrease dimethylation trimethylation lysine 9. Additionally, this phenotype associated interaction deacetylases (HDACs) HP1 (heterochromatin protein 1) repetitive sequences (satellite 2). Our data indicate activity, via appropriate modifications, contributes preservation correct organization large heterochromatic regions.
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