Synthesis of a conjugate vaccine composed of pneumococcus type 14 capsular polysaccharide bound to pertussis toxin.
作者： R Schneerson , L Levi , J B Robbins , D M Bryla , G Schiffman
DOI: 10.1128/IAI.60.9.3528-3532.1992
关键词: Microbiology 、 Immunogenicity 、 Conjugate 、 Biology 、 Conjugate vaccine 、 Bacterial vaccine 、 Immunogen 、 Bordetella pertussis 、 Humoral immunity 、 Pertussis toxin
摘要: Type 14 is one of the common types isolated from patients all ages with infections caused by Streptococcus pneumoniae. Its capsular polysaccharide (Pn14) composed a neutrally charged tetrasaccharide repeat unit. Pn14 does not elicit protective levels antibodies in infants and children less than optimal immunogen 23-valent vaccine for adults. Pertussis toxin (PT) both virulence factor antigen Bordetella pertussis: it soluble at neutral pH forms insoluble complexes acidic polysaccharides. Both PT are potential components vaccines children. Accordingly, synthetic scheme was devised to prepare conjugate PT. An adipic acid hydrazide derivative bound 3.9 carbodiimide-mediated condensation. The conjugation procedure inactivated as assayed CHO cell histamine-sensitizing activity. Pn14-PT elicited mice estimated be humans neutralizing We plan evaluate clinically.
sci-hub.st 参考文章(37)
Stephen I. Morse, Ah Swee Kong, The Effect of Bordetella Pertussis on the Antibody Response in Mice to Type III Pneumococcal Polysaccharide Journal of Immunology. ,vol. 116, pp. 989- 993 ,(1976)
R D Sekura, F Fish, C R Manclark, B Meade, Y L Zhang, Pertussis toxin. Affinity purification of a new ADP-ribosyltransferase. Journal of Biological Chemistry. ,vol. 258, pp. 14647- 14651 ,(1983) , 10.1016/S0021-9258(17)43911-1
D L Burns, J G Kenimer, C R Manclark, Role of the A subunit of pertussis toxin in alteration of Chinese hamster ovary cell morphology. Infection and Immunity. ,vol. 55, pp. 24- 28 ,(1987) , 10.1128/IAI.55.1.24-28.1987
E C Beuvery, F van Rossum, J Nagel, Comparison of the induction of immunoglobulin M and G antibodies in mice with purified pneumococcal type 3 and meningococcal group C polysaccharides and their protein conjugates. Infection and Immunity. ,vol. 37, pp. 15- 22 ,(1982) , 10.1128/IAI.37.1.15-22.1982
H Sato, Y Sato, Bordetella pertussis infection in mice: correlation of specific antibodies against two antigens, pertussis toxin, and filamentous hemagglutinin with mouse protectivity in an intracerebral or aerosol challenge system. Infection and Immunity. ,vol. 46, pp. 415- 421 ,(1984) , 10.1128/IAI.46.2.415-421.1984
J H van de Wijgert, A F Verheul, H Snippe, I J Check, R L Hunter, Immunogenicity of Streptococcus pneumoniae type 14 capsular polysaccharide: influence of carriers and adjuvants on isotype distribution. Infection and Immunity. ,vol. 59, pp. 2750- 2757 ,(1991) , 10.1128/IAI.59.8.2750-2757.1991
M. Granström, K. Hanngren, A.M. Olinder-Nielson, P. Holmblad, A. Mark, Specific immunoglobulin for treatment of whooping cough The Lancet. ,vol. 338, pp. 1230- 1233 ,(1991) , 10.1016/0140-6736(91)92101-7
GAIL BOLAN, CLAIRE V BROOME, RICHARD R FACKLAM, BRIAN D PLIKAYTIS, DAVID W FRASER, WALTER F SCHLECH III, Pneumococcal Vaccine Efficacy in Selected Populations in the United States Annals of Internal Medicine. ,vol. 104, pp. 1- 6 ,(1986) , 10.7326/0003-4819-104-1-1
J. H. Morse, A. S. Kong, J. Lindenbaum, S. I. Morse, The Mitogenic Effect of the Lymphocytosis Promoting Factor from Bordetella Pertussis on Human Lymphocytes Journal of Clinical Investigation. ,vol. 60, pp. 683- 692 ,(1977) , 10.1172/JCI108820
A.E. Churchill, A potency test for inactivated small animal parvovirus vaccines using chicks Journal of Biological Standardization. ,vol. 10, pp. 1- 8 ,(1982) , 10.1016/S0092-1157(82)80042-5