Analysis of fat body transcriptome from the adult tsetse fly, Glossina morsitans morsitans.

摘要: Tsetse flies (Diptera: Glossinidia) are vectors of pathogenic African trypanosomes. To develop a foundation for tsetse physiology, normalized expressed sequence tag (EST) library was constructed from fat body tissue immune-stimulated Glossina morsitans morsitans. Analysis 20,257 high-quality ESTs yielded 6372 unique genes comprised 3059 tentative consensus (TC) sequences and 3313 singletons (available at http://aksoylab.yale.edu). We analysed the putative transcriptome based on homology to other gene products with known functions available in public domain. In particular, we describe immune-related products, reproductive function related yolk proteins milk-gland protein, iron metabolism regulating ferritins transferrin, tsetse's major energy source proline biosynthesis. Expression analysis three indicates that all detected females, while only protein similarity lipases, is males. Milk gland apparently important larval nutrition, however, primarily synthesized by accessory milk tissue.