Whole Genome Analysis of Selected Human Group A Rotavirus Strains Revealed Evolution of DS-1-Like Single- and Double-Gene Reassortant Rotavirus Strains in Pakistan During 2015-2016.

摘要: Acute gastroenteritis due to group A rotaviruses (RVAs) is the leading cause of infant and childhood morbidity mortality particularly in developing countries including Pakistan. In this study we have characterized whole genomes five RVA strains (PAK56, PAK419, PAK585, PAK622, PAK663) using Illumina HiSeq platform. The PAK56 PAK622 exhibited a typical Wa-like genotype constellation (G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1 G3-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1, respectively), whereas PAK663 distinct DS-1-like constellations (G3P[4]-I2-R2-C2-M2-A2-N2-T1-E2-H2, G1P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2, G3P[4]-I2-R2-C2-M2-A2-N2-T2-E2-H2, respectively). Despite their constellation, strain PAK585 possessed G1P[8] genotypes, both PAK419 G3 genotype. addition, also NSP3 T1, suggesting that multiple reassortments occurred. On Phylogenetic analysis, all gene segments examined were genetically related globally circulating human G1, G2, G3, G6, G8, G9, G12 strains. Interestingly, NSP2 showed closest relationship with Indian bovine (India/HR/B91), occurrence reassortment between Furthermore, closely one another most segments, indicating these might been derived from common ancestor. To our knowledge first genome-based molecular characterization rotavirus results will enhance existing on diversity evolutionary dynamics novel intergenogroup reassortant spreading Asian Pakistan, pre-vaccine era. Therefore, continuous surveillance recommended monitor evolution, spread genetic stability such events.