Effects of mutagens on somatic embryogenesis and plant regeneration in groundnut
作者： A. Muthusamy , K. Vasanth , D. Sivasankari , B. R. Chandrasekar , N. Jayabalan
DOI: 10.1007/S10535-007-0092-Y
关键词: Somatic embryogenesis 、 Sodium azide 、 Murashige and Skoog medium 、 Explant culture 、 Biology 、 Horticulture 、 Germination 、 Hypocotyl 、 Embryo 、 Botany 、 Arachis hypogaea
摘要: The embryogenic calli (EC) were obtained from hypocotyl explants of groundnut (Arachis hypogaea L.) cultured on Murashige and Skoog (MS) medium supplemented with different concentrations 2,4-dichlorophenoxyacetic acid (2,4-D) in combination 0.5 mg dm−3 6-benzylaminopurine (BAP). EC exposed to γ-radiation (10–50 Gy) or treated 1–5 mM ethyl methane sulphonate (EMS) sodium azide (SA). mutated subcultured embryo induction containing 20 2,4-D. Somatic embryos (SE) developed these transferred MS BAP (2.0 dm−3) 2,4-D for maturation. well-developed germination consisting salts 2.0 0.25 naphthaleneacetic (NAA). Well-developed plantlets hardening hardened plants produced normal flowers set viable seeds. fresh mass the EC, mean number SE per explant regeneration percentage higher at lower mutagens (up 30 Gy/3 mM). Some abnormalities regenerated observed, especially variations leaf shape.
springer.com
elsevier.com
researchgate.net 
sci-hub.se 参考文章(34)
S. Arunyanart, S. Soontronyatara, Mutation induction by γ and X-ray irradiation in tissue cultured lotus Plant Cell Tissue and Organ Culture. ,vol. 70, pp. 119- 122 ,(2002) , 10.1023/A:1016021627832
U. Laneri, R. Franconi, P. Altavista, Somatic mutagenesis of Gerbera jamesonii hybr.: irradiation and in vitro culture. Acta Horticulturae. pp. 395- 402 ,(1990) , 10.17660/ACTAHORTIC.1990.280.64
Rusli Ibrahim, Wim Mondelaers, Pierre C. Debergh, Effects of X-irradiation on adventitious bud regeneration from in vitro leaf explants of Rosa hybrida. Plant Cell Tissue and Organ Culture. ,vol. 54, pp. 37- 44 ,(1998) , 10.1023/A:1006072205608
B. S. Ahloowalia, In Vitro Radiation Induced Mutagenesis in Potato Springer Netherlands. pp. 39- 46 ,(1990) , 10.1007/978-94-009-0587-0_4
A.K.A. Mandal, D. Chakrabarty, S.K. Datta, Application of in vitro techniques in mutation breeding of chrysanthemum Plant Cell Tissue and Organ Culture. ,vol. 60, pp. 33- 38 ,(2000) , 10.1023/A:1006442316050
L. A. Reddy, K. Vaidyanath, Callus formation and regeneration in two induced mutants of foxtail millet (Setaria italica). Journal of Genetics and Breeding. ,vol. 44, pp. 133- 138 ,(1990)
Bassam Al-Safadi, Zouheer Ayyoubi, Dana Jawdat, The effect of gamma irradiation on potato microtuber production in vitro Plant Cell Tissue and Organ Culture. ,vol. 61, pp. 183- 187 ,(2000) , 10.1023/A:1006477224536
E. L. Little, Z. V. Magbanua, W. A. Parrott, A protocol for repetitive somatic embryogenesis from mature peanut epicotyls. Plant Cell Reports. ,vol. 19, pp. 351- 357 ,(2000) , 10.1007/S002990050739
B. Delbreil, M. Jullien, Evidence for in vitro induced mutation which improves somatic embryogenesis in Asparagus officinalis L. Plant Cell Reports. ,vol. 13, pp. 372- 376 ,(1994) , 10.1007/BF00234140
Charleen M. Baker, Richard E. Durham, J. Austin Burns, Wayne A. Parrott, Hazel Y. Wetzstein, High frequency somatic embryogenesis in peanut ( Arachis hypogaea L.) using mature, dry seed Plant Cell Reports. ,vol. 15, pp. 38- 42 ,(1995) , 10.1007/BF01690250