High prevalence of 16S rRNA methylase RmtB among CTX-M extended-spectrum β-lactamase-producing Klebsiella pneumoniae from Islamabad, Pakistan

摘要: Abstract The aim of this study was to characterise extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae isolated from urinary tract and wound infections Pakistan ( n  = 25). Isolates were subjected commercially available microarray analysis determine the presence ESBLs acquired AmpC enzymes. genetic diversity isolates determined by pulsed-field gel electrophoresis (PFGE) multilocus sequence typing (MLST). Plasmid replicon capsular serotyping conducted PCR. Finally, screening for virulence genes, plasmid-mediated quinolone resistance (PMQR) genes encoding 16S rRNA methylases done using All K. hosted bla CTX-M all strains belonged phylogroup CTX-M-1. Acquired β-lactamases (ACT/MIR CIT group) found in 16% isolates. Two clusters observed with ≥80% similarity among profiles obtained PFGE, two types (STs) MLST, namely ST215 ST307, these clusters. Three carried factor wcaG three had type K20. IncFIA, IncFIB, IncFIIK FrepB replicons most commonly collection. Among PMQR determinants, aac(6 ′ )-lb - cr present 96% (24/25) isolates, qnrB 88% (22/25) qepA 4% (1/25). methylase-encoding gene rmtB 60% (15/25) In conclusion, CTX-M-producing ST307 major clones detected. Of particular concern high prevalence conferring aminoglycosides.